Genetic Monitoring Program
Genetic monitoring is key to quality control of the breeding and production of inbred and transgenic lines of laboratory mice and rats. Taconic has established numerous production procedures that minimize the risk of genetic contamination. Our Genetic Monitoring Program is grounded in the identification of points in the production program where there is a higher risk of an undetected accidental mating or strain misidentification. By focusing genetic screening methods on these critical high-risk points, while scrutinizing quality control throughout, a genetic monitoring program can prevent a single mistake from becoming catastrophic.
Taconic's overall Genetic Monitoring Program is based on a combination of three strategies:
Visual characteristics of strains (most notably, coat color)
Production procedures that minimize co-localization and/or movement of strains of similar colors and that promote careful record keeping and strain segregation
Standard operating procedures outlining DNA-based marker and/or protein testing of animals at specific, defined, potentially risky points in the production process.
Markers and Assays
Taconic currently uses Single Nucleotide Polymorphisms (SNPs) and genotyping in its Genetic Monitoring Program.
More specifically, the program uses the Illumina platform and has developed several SNP Panels for Genetic Monitoring of rats and mice. Including a C57BL/6 96 SNP Panel to differentiate between C57BL/6N and C57BL/6J sub-strains.