SPECIFICATIONS OF THE NIH-31M DIET

The natural ingredient NIH #31M Rodent Diet is used as the standard diet in rodent production colonies at all US Taconic production sites. This diet formulation is a modification of the widely used NIH #31M Rodent Diet with the energy concentration increased by slightly increasing the percentage of soybean oil in the formulation. This is an open formula diet and the quantitative ingredient composition is presented in the table below.

Vendors providing the NIH #31M Rodent Diet to Taconic are required to provide a record of the amount of each ingredient used to manufacture every batch of feed shipped to Taconic. Vendors also provide the results of a proximate analyses conducted on a representative feed sample collected from the shipment. This information is used to document the quality of feed provided by the manufacturer. In addition, representative feed samples are periodically collected at Taconic and sent to an independent laboratory to be assayed for various vitamins, minerals, heavy metals, and chemical contaminants.

Ingredient Percentage by Weight
Ground whole wheat 35.17
Ground whole yellow corn 20.00
Ground whole oats 10.00
Wheat middlings 10.00
Fish meal (60% protein) 9.00
Soybean meal (47.5% protein) 5.00
Soy bean oil (no additives) 2.50
Alfalfa meal (17% protein) 2.00
Corn gluten meal (60% protein) 2.00
Dicalcium phosphate 1.50
Brewer’s dried yeast 1.00
Ground limestone 0.50
Salt 0.50
NIH #31 vitamin premix 0.25
NIH #31 mineral premix 0.25
Choline chloride 0.13
L-lysine 0.10
DL-methionine 0.10
Vitamin Amount Source
Vitamin A 24,300,000 IU Stabilized Vitamin A Palmitate or Acetate
Vitamin D 4,200,000 IU D-Activated animal sterol
Vitamin K 22.0 g Menadione activity
Vitmain E 16.5 g dl alpha Tocopherol Acetate
Biotin 132.0 mg d-Biotin
Folic acid 1.1 g  
Niacin 22.0 g  
Pantothenic acid 27.5 g d-Calcium pantothenate
Pyridoxine 2.2 g Pyridoxine hydrochloride
Riboflavin supplement 5.5 g  
Thiamine 71.5 g Thiamine mononitrate
Vitamin B12 supplement 15,400.0 μg  
Mineral Amount Source
Cobalt 440.0 mg Carbonate
Copper 4.4 g Copper Sulfate
Iron 66.0 g Iron sulfate
Magnesium 440.0 g Magnesium oxide
Manganese 110.0 g Manganese oxide
Zinc 11.0 g Zinc oxide
Iodine 1.7 g Calcium iodate
Nutrient Minimum Maximum
Crude protein 18.00% 19.54%
Crude fat 4.98% 6.08%
Crude fiber 3.28% 5.00%
Ash 5.17% 8.00%
Moisture 8.00% 13.00%
Amino acids % of Total Diet
Arginine 1.01
Lysine .99
Methionine .46
Cystine .27
Tryptophan .20
Histidine .41
Leucine 1.47
Isoleucine .79
Phenylalanine .82
Tyrosine .63
Threonine .67
Valine .90
Minerals Amount
Calcium 1.11%
Phosphorous .93%
Potassium .57%
Sodium .28%
Magnesium .22%
Iron 206 mg/kg
Zinc 58 mg/kg
Manganese 152 mg/kg
Copper 17 mg/kg
Cobalt 0.7 mg/kg
Iodine 1.97 mg/kg
Vitamins Amount
Vitamin A 25.90 IU/g
Vitamin D3 4.1 IU/gm
Thiamine 76.90 mg/kg
Riboflavin 7.6 mg/kg
Niacin 82.80 mg/kg
Pantothenic acid 38.10 mg/kg
Choline 1880 mg/kg
Pyridoxine 10.60 mg/kg
Folic acid 1.56 mg/kg
Biotin .29 mg/kg
Vitamin B 12 56 mcg/kg
Vitamin K 22 mg/kg
Vitamin E 46.80 mg/kg
Calories provided by: Amount
Protein 20.940%
Fat (ether extract) 13.736%
Carbohydrates 65.324%