B6 Physiological Data Summary - February, 2004

Phenotyping Procedures:

Animal Receipt and Maintenance

A quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line C57BL/6 (C57BL/6NTac), age 7 weeks. Mice were received at RBU 3, Taconic, Albany NY and acclimated for three days on irradiated NIH #31M rodent diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1-10, females 11-20).

Urinalysis

All mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer's instructions.

Clinical Chemistry and Hematology

A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood, and slides were delivered to LabCorp (RTP, NC) for analysis. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually.

Necropsy and Organ Weights

All mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. The pathologist's summary and detailed histological descriptions follow.

Discussion

All animals were thrifty on arrival and appeared clinically normal. Locomotor behavior was normal and there were no visible lesions or discharges at the mucous membranes. All tissues appeared normal on gross necropsy evaluation and weights were within normal limits. There were no unusual findings upon collection of these tissues nor otherwise observed in the body cavities.

The overall profile of these mice is consistent with expectations of a general-purpose mouse. None of the parameters are indicative of impaired system function.

Parameter Units C57BL/6 Males C57BL/6 Females

Serum Chemistry

Avg ± S.D.    
Calcium mg/dL 10.4 ± 0.8 10.3 ± 0.5
Phosphorous mg/dL 10.5 ± 1.1 10.2 ± 1.2
Glucose mg/dL 167 ± 29 155 ± 15
Creatinine mg/dL N/a N/a
BUN mg/dL 25 ± 4 25 ± 4
Total Bilirubin mg/dL N/a N/a
ALK U/L 165 ± 17 259 ± 58
ALT U/L U/L 86 ± 160 90 ± 150
Total Protein g/dL 6.1 ± 0.4 6.2 ± 0.3

Blood Counts

Erythron      
Red Blood Cells X10^6/uL 8.364 ± 0.610 9.894 ± 0.436
Nucleated RBC X10^6/uL 0 ± 0 0 ± 0
Hemoglobin g/dL 13.1 ± 0.9 15.2 ± 0.6
Hematocrit % 41.8 ± 3.3 48.8 ± 1.8
MCV fL 50 ± 2 49 ± 1
MCH pG 15.7 ± 0.3 15.4 ± 0.3
MCHC % 31.5 ± 0.7 31.2 ± 0.4
Platelets      
Platelets X10^3/uL 1775 ± 171 1336 ± 82
Leukogram      
White Blood Cells x10^3/uL 6.614 ± 2.626 4.543 ± 1.837
Neutrophil x10^3/uL 1.276 ± 0.639 0.407 ± 0.255
Bands x10^3/uL 0 ± 0 0 ± 0
Lymphocyte x10^3/uL 5.182 ± 2.081 4.082 ± 1.622
Monocytes x10^3/uL 0.156 ± 0.178 0.054 ± 0.082
Eosinophil x10^3/uL 0 ± 0 0 ± 0
Basophils x10^3/uL 0 ± 0 0 ± 0
Others x10^3/uL 0 ± 0 0 ± 0

Organ Weights

     
Stomach g 0.209 ± 0.049 0.169 ± 0.028
Ileum g 0.608 ± 0.080 0.587 ± 0.056
Colon g 0.164 ± 0.026 0.145 ± 0.019
Lungs g 0.169 ± 0.025 0.180 ± 0.021
Heart g 0.108 ± 0.015 0.111 ± 0.014
Liver g 0.924 ± 0.067 0.834 ± 0.088
Spleen/Pancreas g 0.155 ± 0.024 0.165 ± 0.022
Kidney (L) g 0.106 ± 0.011 0.111 ± 0.011
Kidney (R) g 0.118 ± 0.011 0.114 ± 0.009
Testes (L) g 0.057 ± 0.006 -
Testes (R) g 0.056 ± 0.006 -
Ovary (L) g - 0.013 ± 0.003
Ovary (R) g - 0.015 ± 0.003

Urinalysis

     
Glucose mg/dL N/a N/a
Blood - N/a N/a
Ph - 6.1 ± 0.2 6.1 ± 0.2
Protein mg/dL N/a N/a
Specific Gravity - 1.019 ± 0.003 1.026 ± 0.002