The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Vnn1 exon 1 contains the translation initiation codon.
Exon 4 has been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 4 should result in loss of function of the Vnn1 gene by deleting the C-term of Carbon-nitrogen hydrolase domain.
The remaining recombination sites are located in intron regions of the genome.
Data mining and Design performed in 2006.
Gene Family: Hydrolase
Genetic Background: C57BL/6
Gene Symbol: Vnn1
Gene Accession Number: NM_011704
Allele Type: KO
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: vanin 1