The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic's C57BL/6N Tac ES cell line.
Tnfrsf18 exon 1 contains the translation initiation codon.
Exons 1 to 5 have been replaced by the FRT site flanked neomycin resistance gene cassette.
The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
The constitutive KO line has been derived using C57BL/6NTac animals.
Bud31 (Edg2) constitutive KO mice are in a mixed C57BL/6-129SvEv genetic backgroundDeletion of exons 1 to 5 should result in loss of function of the Tnfrsf18 gene.
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2004.
Gene Family: Receptor
Genetic Background: C57BL/6
Gene Symbol: Tnfrsf18
Gene Accession Number: NM_009400
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: tumor necrosis factor receptor superfamily, member 18