TNF-α - Model 1921

Constitutive Knock Out

TNF-α (Model 1921) Constitutive Knock Out Mouse Model
This line is cryopreserved and requires cryorecovery.

  • Model #
  • Genotype
  • Nomenclature
  • 1921-F
    ko/ko
    B6.129-TNFtm1Ljo N7
  • 1921-M
    ko/ko
    B6.129-TNFtm1Ljo N7
A replacement-type targeting vector designed to inactivate the TNF gene was constructed. The vector contains a 2.5 kb fragment from the 5´-flanking region of the mouse TNF locus (which includes the LT-alpha gene coding sequence), a PGK-neo expression cassette, and an ~1 kb fragment spanning exons 3 and 4 of the TNF gene. Integration of the targeting vector results in the deletion of a 2.2 kb fragment containing the promoter region, transcriptional start site, and exons 1 and 2 of the TNF gene. These exons encode a portion of the mature TNF domain as well as the leader sequence, a highly conserved region required for proper intracellular processing.

Origin:

The TNF-α knockout mouse was developed by Michael Marino in the laboratory of Lloyd J. Old, Director of the LICR-New York Branch in collaboration with the LICR-Melbourne Branch in Australia. The model was created by targeting the Tnf gene in W9.5 ES cells and injecting the targeted cells into blastocysts. The line was backcrossed 7 generations to C57BL/6J. Taconic received stock for the Emerging Models program in 2001. The model was embryo transfer derived. The model is maintained through incrossing of homozygous mice.


Color:

Black

Species:

Mouse

Initial Publication:

Marino MW, Dunn A, Grail D, Inglese M, Noguchi Y, Richards E, Jungbluth A, Wada H, Moore M, Williamson B, Basu S, Old LJ. Characterization of tumor necrosis factor-deficient mice. Proc Natl Acad Sci U S A. 1997 94(15):8093-8.

Wada H, Noguchi Y, Marino MW, Dunn AR, Old LJ. T cell functions in granulocyte/macrophage colony-stimulating factor deficient mice. Proc Natl Acad Sci U S A. 1997 94(23):12557-61.

Elkon KB, Liu CC, Gall JG, Trevejo J, Marino MW, Abrahamsen KA, Song X, Zhou JL, Old LJ, Crystal RG, Falck-Pedersen E. Tumor necrosis factor alpha plays a central role in immune-mediated clearance of adenoviral vectors. Proc Natl Acad Sci U S A. 1997 94(18):9814-9.

Noguchi Y, Wada H, Marino MW, Old LJ. Regulation of IFN-gamma production in granulocyte-macrophage colony-stimulating factor-deficient mice. Eur J Immunol. 1998 (12):3980-8.

Doi TS, Marino MW, Takahashi T, Yoshida T, Sakakura T, Old LJ, Obata Y. Absence of tumor necrosis factor rescues RelA-deficient mice from embryonic lethality. Proc Natl Acad Sci U S A. 1999 96(6):2994-9.


This mouse strain (including any and all progeny, "MICE") is distributed by Taconic with the following terms and conditions.

Non-Profit Users ("Recipient"): Non-profit users may access the line subject to the Conditions of Use below. The line is available for immediate cryorecovery for non-profit users; cryorecovery fees are additional.
  • MICE will be used for research use and not for a Commercial Purpose.
  • MICE will not be sold or distributed to third parties.
  • Recipient will acknowledge Ludwig Institute for Cancer Research as the source of the MICE in all publications containing any data or information about the MICE.
  • The MICE are experimental in nature and they are provided WITHOUT ANY WARRANTIES, EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR USE. TACONIC AND LUDWIG INSTITUTE FOR CANCER RESEARCH MAKE NO REPRESENTATION AND PROVIDE NO WARRANTY THAT THE USE OF THE MICE WILL NOT INFRINGE ANY PATENT OR OTHER PROPRIETARY RIGHT.
  • To the extent allowable under applicable laws, Recipient agrees to indemnify, defend, and hold harmless Taconic and Ludwig Institute for Cancer Research and its trustees, officers, staff, representatives, and agents against all damages, expenses (including without limitation legal expenses), claims, demands, suits or other actions arising from Recipient's acceptance, use, and disposal of the MICE, and their progeny or derivatives.
For-Profit Users ("Recipient"): Use of MICE by for-profit entities or for Commercial Purposes requires a license from The Ludwig Institute for Cancer Research prior to shipping. Please contact Intellectual Property Manager (LINK TO EMAILS ip@licr.org and nbulgin@licr.org) at the Ludwig Institute For Cancer Research Ltd. Once a license has been executed, Taconic may cryorecover the line. Cryorecovery fees and handling/maintenance fees are additional.

Commercial Purpose means: uses of the MICE by any organization, including Recipient to perform contract research, screen compound libraries, produce or manufacture products for general sale, or to conduct research activities that result in sale, lease, licenses or transfer of the MICE to a for-profit organization. For clarity, industrially sponsored academic research shall be considered a use of the MICE for Commercial Purposes.

In no event shall Taconic or the Ludwig Institute for Cancer Research be liable for any use of the MICE, and Recipient hereby agrees to defend, indemnify and hold Taconic and the Ludwig Institute for Cancer Research harmless from any loss, claim, damage, or liability which may arise from Recipient's use, storage and disposal of the MICE.