The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 5 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.0 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 5 and 6 should result in loss of function of the Tbk1 gene by deleting part of the catalytic domain and by generating a frameshift from exon 4 to exons 7 to 9 (premature Stop codon in exon 7).
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, the Xpot gene (ENSMUSG00000034667; EntrezGene 73692) is located approx. 15 kb upstream of Tbk1 exon 5, in tail-to-head orientation.
Datamining and Design performed in 2010.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Tbk1
Gene Accession Number: NM_019786.3
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: TANK-binding kinase 1