Pxr Knockout Mouse

Constitutive Knock Out

Pxr Constitutive Knock Out Mouse Model

  • Model #
  • Genotype
  • Nomenclature
  • 9100-F
    ko/ko
    C57BL/6-Nr1i2tm3Arte
  • 9100-M
    ko/ko
    C57BL/6-Nr1i2tm3Arte
  • This targeted mutation strain carries a deletion of the mouse Nr1i2 gene which encodes for the nuclear receptor PXR.
  • Useful in defining the effect of PXR on CYP induction and therefore pharmacokinetics, drug toxicity, and efficacy.

Origin:

The Pxr Knockout mouse was developed by Taconic in collaboration with CXR Biosciences. It was generated by crossing a PXR humanized mouse line with a PhiC31 deleter mouse. The Humanized PXR Mouse model was created through a knock in of a human PXR cDNA/genomic construct onto the ATG of murine Pxr in C57BL/6NTac-derived ES cells. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate the selection marker. The Pxr Knockout Mouse model was derived from the Humanized PXR Mouse line through a PhiC31-mediated deletion of the human PXR sequence. Mouse exon 2, carrying the translational start site, is deleted in the knockout, while mouse exon 1 and exons 3-9 are still present. A splice acceptor polyA motif included in the targeting vector causes splicing of exon 1 to a polyA motif and thereby terminates the transcription. The absence of PXR protein was proven experimentally. Taconic received stock in 2009, and the line was embryo transfer derived. The colony is maintained by mating homozygotes.


Color:

Black

Species:

Mouse

Initial Publication:

Scheer N, Ross J, Rode A, Zevnik B, Niehaves S, Faust N, Wolf CR. (2008) A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response. J. Clin. Invest. 118(9): 3228-3239.



Label License: Conditions of Use - Model 9100
This Model is produced and distributed under rights to patents and intellectual property owned by Taconic or licensed from various institutions. Taconic sells this model to purchaser, and grants to each purchaser a right under Taconic’s own or sublicensable rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers’ acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:

  • Title to this Model and biological materials derived from it remains with Taconic Biosciences, Inc.
  • The Model will be used for research purposes only.
  • The Model will not be bred except to obtain embryos or fetuses required for research purposes.
  • The Model and biological materials derived from it will not be distributed to third parties or used for commercial purposes.
  • Nonprofit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.