Humanized PXR Mouse

Targeted Replacement

Humanized PXR Mouse Targeted Replacement Mouse Model

  • Model #
  • Genotype
  • Nomenclature
  • 9104-F
  • 9104-M
  • This targeted mutation strain carries a replacement of the mouse Nr1i2 gene which encodes for the nuclear receptor PXR with the corresponding human orthologous gene.
  • Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity, and efficacy in humans.


The Humanized PXR Mouse was developed by Taconic in collaboration with CXR Biosciences. It was created through a knock in of a human PXR cDNA/genomic construct onto the ATG of murine Pxr in C57BL/6NTac-derived ES cells. The human PXR sequence that was introduced contains a cDNA fusion of exon 2-4, intron 4, exon 5, intron 5, exon 6, intron 6, exon 7, intron 7, and cDNA fusion exon 8 + 9. The expression of the human PXR receptor is driven by the mouse Pxr promoter. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate the selection marker. Taconic received stock in 2009, and the line was embryo transfer derived. The colony is maintained by mating homozygotes.





Initial Publication:

Scheer N, Ross J, Kapelyukh Y, Rode A, Wolf CR. (2010). In vivo responses of the human and murine pregnane X receptor to dexamethasone in mice. DMD 38(7): 1046-1053.

Label License: Conditions of Use - Model 9104
This Model is produced and distributed under rights to patents and intellectual property owned by Taconic or licensed from various institutions. Taconic sells this model to purchaser, and grants to each purchaser a right under Taconic’s own or sublicensable rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers’ acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:

  • Title to this Model and biological materials derived from it remains with Taconic Biosciences, Inc.
  • The Model will be used for research purposes only.
  • The Model will not be bred except to obtain embryos or fetuses required for research purposes.
  • The Model and biological materials derived from it will not be distributed to third parties or used for commercial purposes.
  • Nonprofit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.