The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6J ES cell line.
Plk1 exon 1 contains the translation initiation codon.
Exons 4-5 have been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 4-5 should result in loss of function of the Plk1 gene by deleting most of the kinase domain.
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2005.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Plk1
Gene Accession Number: NM_008401
Allele Type: cKO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: polo-like kinase 1 (Drosophila)