The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Pkmyt1 exon 2 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
Deletion of exon3 should result in loss of function of the Pkmyt1 gene by generating a frameshift (premature stop codon in exon 4).
The remaining recombination sites are located in intron regions of the genome.
Data mining and Design performed in 2005.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Pkmyt
Gene Accession Number: NM_023058
Allele Type: cKO
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: protein kinase, membrane associated tyrosine/threonine 1