The targeting vector has been generated using C57BL/6J DNA and has been transfected into TaconicArtemis C57BL/6N Tac ES cell line.
Npr3 exon 1 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 0.8 kb).
The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
Deletion of exon 3 should result in the loss of function of the Npr3 gene by deleting part of the extracellular domain and by generating a frameshift from exon 2 to exon 4 (premature Stop codon in exon 4).
The remaining recombination sites are located in non-conserved regions of the genome.
The corresponding constitutive KO allele is available as line 13233.
Datamining and Design performed in 2013.
Gene Family: Receptor
Genetic Background: C57BL/6
Gene Symbol: Npr3
Gene Accession Number: NM_008728.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: natriuretic peptide receptor 3