Lpin3 - Model 10584 - cKO

Conditional Knock Out

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 10584
    C57BL/6-Lpin3tm1.1 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 2 contains the translation initiation codon.
  • Exons 4 to 7 have been flanked by loxP sites (size of loxP-flanked region: 2.9 kb).
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 4 to 7 should result in loss of function of the Lpin3 gene by deleting the C-terminal part of the Lipin domain and the potential nuclear localization signal and by generating a frameshift from exon 3 to exons 8-11 (premature Stop codon in exon 8).
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • According to NCBI and Ensembl databases, there are two genes in the neighborhood of the Lpin3 gene:

    • The Zhx3 gene (Entrez ID: 320799; ENSMUSG00000035877) is located approx. 22 kb upstream of Lpin3 exon 4 in a head-to-head orientation.
    • The Emilin3 gene (Entrez ID: 280635; ENSMUSG00000050700) is located approx. 9 kb downstream of Lpin3 exon 7 in a tail-to-tail orientation.

  • The corresponding constitutive KO allele is available as line 10615.
  • Datamining and Design performed in 2009.

Gene Family:


Genetic Background:


Gene Symbol:


Gene Accession Number:


Allele Type:





Cryopreserved at Taconic



NOTICE: Access to this model is conditioned on the requestor's institution agreeing to the terms and conditions of a Material Transfer Agreement (MTA).

Steps to initiate and place order:

  1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
  2. Nonprofit Investigator inquires on Taconic website.
  3. Taconic will answer questions about the GEM lines and the process.
  4. Taconic sends request for MTA.
  5. Nonprofit Investigator provides scientific workplan answering 4 basic questions.
  6. MTA is issued for Nonprofit Investigator/institution review and signature.
  7. MTA is executed by all parties and release of GEM is approved.
  8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and shipment costs.
  9. Taconic begins cryorecovery upon receipt of PO and delivers GEM line to requestor (See pricing and Deliverables).

It is the requestor's obligation to determine that the GEM meets their research needs.

Learn more about Terms of Use, The Scientific Workplan and Frequently Asked Questions.
The KO Repository lines exist as cryopreserved materials. Cohorts can typically be produced and delivered to the customer within 14-16 weeks of order receipt. For most lines, the mouse ES cells used in generating the model are of the 129S5 strain. Lines are stored primarily as cryopreserved sperm on a mixed 129S5 x C57BL/6J-Tyrc-Brd background. Lines are recovered using cryopreserved sperm and C57BL/6NTac oocyte donors. Some lines are stored as cryopreserved embryos and when revitalized will result in a 129/S5 x C57BL/6J-Tyrc-Brd background.

For standard orders clients will receive:

  • A minimum of four (4) Mutant Mice, heterozygous for the Selected Mutation, including at least one (1) breeding pair on a mixed 129S5;B6 background.
  • Validated genotyping protocols
  • Animal health data