Humanized Liver CYP2C9 Mouse

Targeted Replacement

Humanized Liver CYP2C9 Targeted Replacement Mouse Model

  • Model #
  • Genotype
  • Nomenclature
  • 11746-F
    C57BL/6-Is(19Alb/CYP2C9;Del 19Cyp2c55-Cyp2c70)2Arte
  • 11746-M
    C57BL/6-Is(19Alb/CYP2C9;Del 19Cyp2c55-Cyp2c70)2Arte
  • This model carries a replacement of fourteen full-length mouse Cyp2c genes (Cyp2c55, 2c65, 2c66, 2c29, 2c38, 2c39, 2c67, 2c68, 2c40, 2c69, 2c37, 2c54, 2c50, 2c70) with an expression cassette encoding human CYP2C9 under control of a liver specific albumin promoter.
  • CYP2C9 catalyzes the metabolism of many drugs in clinical use
  • May be used as a bioreactor to generate human-specific metabolites, to determine the relevance of CYP2C9 in drug metabolism or for CYP2C9 inhibition studies.
  • This model may be used in combination with a Cyp2c knockout model (9177) in order to determine the contribution of CYP2C9-mediated drug metabolism.

Genetic Background:

C57BL/6 Background


The Humanized Liver CYP2C9 Mouse model was developed by Taconic in collaboration with CXR Biosciences. The model was created by four consecutive rounds of transfection in C57BL/6NTac-derived ES cells. First, mouse exon 1 of Cyp2c55 was replaced with a neomycin cassette containing a loxP and f3 site through gene targeting. Second, a hygromycin cassette with a loxP, lox5171 and frt site was inserted into intron 6 of the Cyp2c70 gene through gene targeting. Third, transfection of the ES cells with cre recombinase resulted in the deletion of the Cyp2c55, 2c65, 2c66, 2c29, 2c38, 2c39, 2c67, 2c68, 2c40, 2c69, 2c37, 2c54, 2c50 and 2c70 genes. Fourth, an albumin-CYP2C9 expression cassette was inserted by cre-mediated recombination into the loxP and lox5171 sites in the mouse Cyp2c locus. The targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate selection markers. The colony is maintained by mating homozygotes.





Initial Publication:

Scheer N, Kapelyukh Y, Chatham L, Rode A, Buechel S, Wolf CR. Generation and Characterization of Novel Cytochrome P450 Cyp2c Gene Cluster Knockout and CYP2C9 Humanized Mouse Lines. Mol Pharmacol. 2012 Aug 23.

Label License: Conditions of Use - Model 11746
This Model is produced and distributed under rights to patents and intellectual property owned by Taconic or licensed from various institutions. Taconic sells this model to purchaser, and grants to each purchaser a right under Taconic’s own or sublicensable rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers’ acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:

  • Title to this Model and biological materials derived from it remains with Taconic Biosciences, Inc.
  • The Model will be used for research purposes only.
  • The Model will not be bred except to obtain embryos or fetuses required for research purposes
  • The Model and biological materials derived from it will not be distributed to third parties or used for commercial purposes
  • Nonprofit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.