The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 2 to 5 have been flanked by loxP sites (size of loxP-flanked region: 1.7 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 2 to 5 results in loss of function of the Lcat gene by deleting part of the catalytic domain (including the active site).
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, there are 3 genes in the neighborhood of the Lcat gene:
- The Slc12a4 gene (Entrez ID: 20498; ENSMUSG00000017765) is located 1 kb upstream of Lcat exon 2 in a tail-to-head orientation.
- The Psmb10 gene (Entrez ID: 19171; ENSMUSG00000031897) is located 3.5 kb downstream of Lcat exon 5 in a tail-to-head orientation.
The corresponding constitutive KO allele is available as line 11114.
Datamining and Design performed in 2010.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Lcat
Gene Accession Number: NM_008490.1
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: lecithin cholesterol acyltransferase