Klrd1 - Model 12666 - KO

Constitutive Knock Out

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

  • Model #
  • Genotype
  • Nomenclature
  • 12666
  • The targeting vector has been generated using 129 DNA and transfected into 129 ES cell line.
  • Klrd1 exon 3 has been disrupted and exon 4 removed by the neomycin resistance cassette, which is flanked by loxP sites.
  • The neomycin resistance gene has been deleted by transient transfection of Cre in targeted ES cells.
  • Chimeric mice were produced and germ line transmission achieved.
  • The constitutive KO line has been subsequently backcrossed to C57BL/6 animals for 9 generations.
  • Deletion of parts of exon 3 and entire exon 4 should result in loss of function of the Klrd1 gene by deleting a dimerization and the C-type lectin-like domains.
  • The remaining recombination sites are located in intron regions of the genome.
  • See M.T. Orr et al, PLoS ONE 5, e15184, 2010, for further details.

Gene Family:


Genetic Background:


Gene Symbol:


Gene Accession Number:


Allele Type:





Cryopreserved at Taconic



Official Gene Name:

killer cell lectin-like receptor, subfamily D, member 1

NOTICE: Access to this model is conditioned on the requestor's institution agreeing to the terms and conditions of a Material Transfer Agreement (MTA).

Steps to initiate and place order:

  1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
  2. Nonprofit Investigator inquires on Taconic website.
  3. Taconic will answer questions about the GEM lines and the process.
  4. Taconic sends request for MTA.
  5. Nonprofit Investigator provides scientific workplan answering 4 basic questions.
  6. MTA is issued for Nonprofit Investigator/institution review and signature.
  7. MTA is executed by all parties and release of GEM is approved.
  8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and shipment costs.
  9. Taconic begins cryorecovery upon receipt of PO and delivers GEM line to requestor (See pricing and Deliverables).

It is the requestor's obligation to determine that the GEM meets their research needs.

Learn more about Terms of Use, The Scientific Workplan and Frequently Asked Questions.
Upon execution of the MTA terms of use, requestor needs to provide purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and additional shipment costs.

  1. Taconic will begin standard derivation of cryopreserved material (sperm or embryos) upon receipt of PO from recipient.
  2. Taconic will conduct testing using its International Health Monitoring System™ (IHMS™) to ensure that the mice meet a Murine Pathogen Free™ (MPF™) health status.
  3. Taconic will genotype the recovered mice prior to shipment.
  4. Taconic will provide a health status report, the genotyping results of the recovered animals and genotyping assay information for the requested GEM line.
  5. Taconic shall deliver a minimum of two (2) mice heterozygous at the selected gene of interest of a requested GEM line.
  6. Taconic will make shipping arrangements for the requested GEM to the Licensed Nonprofit recipient.
  7. Nonprofit investigator is responsible for covering the shipment costs.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.