The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 1.1 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 3 should result in loss of function of the Kctd10 gene by deleting part of the BTB/POZ-like domain and by generating a frameshift to all downstream exons.
According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Kctd10 gene:
- The Ube3b gene (NCBI ID: 117146, Ensembl: ENSMUSG00000029577) is located approx. 10 kb upstream of Kctd10 exon 3 in a head-to-head orientation.
- The Myo1h gene (NCBI ID: 231646, Ensembl: ENSMUSG00000066952) is located approx. 6.5 kb downstream of Kctd10 in a tail-to-tail orientation.
The corresponding constitutive KO allele is available as line 9991.
Datamining and Design performed in 2008.
Gene Family: Phosphoprotein
Genetic Background: C57BL/6
Gene Symbol: Kctd10
Gene Accession Number: NM_026145.3
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: potassium channel tetramerisation domain containing 10