IL15 Knockout

Constitutive Knock Out

IL15 Constitutive Knock Out Mouse Model
EZcohort™ Models
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C57BL/6NTac Background

  • Model #
  • Genotype
  • Nomenclature
  • 4269-F
    C57BL/6NTac-IL15tm1Imx N5
  • 4269-M
    C57BL/6NTac-IL15tm1Imx N5
IL-15 is a member of the IL-2 family of cytokines that stimulates proliferation of activated T cells, NK cells, and B cells through a receptor complex containing a high affinity ligand binding subunit, IL-15Ra and the Jak/STAT signaling elements IL-2Rb and g c. In addition IL-15 stimulates cytolytic effector function and facilitates the survival of CD8 memory T cells. IL-15 deficient mice lack natural killer (NK) cells and have marked reductions in memory CD8 T cells, NK1.1 T cells, and Thy1- CD8a intraepithelial lymphocytes (IELs). These mice are unable to mount a protective immune response to challenge with vaccinia virus. They should be useful for exploring the role of IL-15 in other host immune responses.

Genetic Background:

C57BL/6 Background

Origin:

C57BL/6 mice deficient in IL15 were generated by gene targeting in the laboratory of Dr. Jacques Peschon of the Immunex Corporation in 1996. The IL15 gene was disrupted by replacing a 7.5 kb fragment containing IL15 exons 3-5 with a PGK-neo cassette. The disrupted gene was introduced into ES cells of C57BL/6 origin developed at Immunex and injected into BALB/c blastocysts to create chimeric mice. Resulting male chimeric animals were bred to C57BL/6 females and offspring heterozygous for the IL15 knockout allele were crossed to generate IL15 deficient mice (IL15-/-). Different C57BL/6 sublines were used during the development of this line and therefore an exact control mouse was not available. However, C57BL/6NTac mice were used as controls in the original studies. Recently we completed backcrossing these mice an additional 5 times onto C57BL/6NTac mice and intracrossed them to make them homozygous for the IL15 knockout allele. We now consider the Taconic B6 (C57BL/6NTac) mouse to be a more appropriate control for line 4269.

Color:

Black

Species:

Mouse

Initial Publication:

Kennedy MK, Glaccum M, Brown SN, Butz EA, Viney JL, Embers M, Matsuki N, Charrier K, Sedger L, Willis CR, Brasel K, Morrissey PJ, Stocking K, Schuh JCL, Joyce S and Peschon JJ. 2000. Reversible defects in natural killer and memory CD8 T cell lineages in interleukin 15-deficient mice. J Exp Med. 2000 Mar 6;191(5):771-80

Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.