hIL15-NOG

hIL15-NOG Mouse Model

NOD Background

  • Model #
  • Genotype
  • Nomenclature
  • 13683-F
    sp/sp;ko/ko;tg/wt
    NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(CMV-IL2/IL15)1-1Jic/JicTac
  • 13683-M
    sp/sp;ko/y;tg/wt
    NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(CMV-IL2/IL15)1-1Jic/JicTac
  • Super immunodeficient NOG mouse expressing human IL-15 cytokine
  • Predominant differentiation of human NK cells following human HSC engraftment compared to the base NOG mouse, but survival time is limited
  • Human NK cells developed in hIL15-NOG mice following HSC engraftment express various NK receptors and produce both granzyme A and perforin upon stimulation
  • Engraftment and expansion of human NK cells following engraftment with CD56+ NK cells derived from PBMCs
  • May be useful for efficacy studies involving antibody therapeutics with antibody dependent cell cytotoxcity mechanism of action
  • Applications in research involving cancer, infectious disease, immunology, regenerative medicine and human immune system engraftment

Origin:

The CIEA NOG mouse® was developed by Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. The Prkdc scid mutation was identified by Mel Bosma of the Fox Chase Cancer Center in a C.B-17 congenic mouse population. This mutation was backcrossed onto the NOD/ShiJic strain at CIEA for at least eight generations. The Il2rg targeted mutation was developed by Dr. Kazuo Sugamura of Tohoku University by targeting the gene in ES cells derived from a 129 strain. Portions of exons 7 and 8 were replaced with a neo cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Resultant chimeras were backcrossed onto the C57BL/6JJic background for at least eight generations. The CIEA NOG mouse® was developed by backcrossing the C57BL/6JJic-Il2rg line to the NOD/ShiJic-Prkdc scid line for a total of eight generations. The hIL15-NOG mouse was developed by microinjecting a transgene consisting of a DNA fragment containing human IL-15 cDNA with a hIL-2 signal peptide, under the control of a CMV-promoter, into zygotes of NOD/ShiJic-Il2rg mice.  Founders were backcrossed to NOG mice to establish the hIL15-NOG line.  Taconic received stock in 2014, and the line was derived through embryo transfer. 


Availability:

Available now*
*Please note that advance order is encouraged for order of male mice. There is a minimal 4-week advance notice required on orders of male older than 3 weeks of age.

Color:

Albino

Species:

Mouse

Initial Publication:

Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, Heike T, Nakahata T. (2002) NOD/SCID/γ mouse: an excellent recipient mouse model for engraftment of human cells. Blood 100(9):3175-3182.

Fukuchi Y, Miyakawa Y, Kobayashi K, Kuramochi T, Shimamura K, Tamaoki N, Nomura T, Ueyama Y, Ito M. (1998) Cytokine dependent growth of human TF-1 leukemic cell line in human GM-CSF and IL-3 producing transgenic SCID mice. Leuk Res 22(9):837-43.

*The hIL15-NOG mouse has not been published yet. Initial publications above refer to the core NOG mouse.



Nonprofit users (excluding users at nonprofit foundations which are affiliated with a for-profit entity): For internal research purposes, the hIL15-NOG Conditions of Use for nonprofit users apply. If you wish to perform sponsored research or fee-for-service contract research using this line, you must purchase under the for-profit terms and pricing.

For-profit users and users at foundations which are affiliated with for-profit entities: The hIL15-NOG Conditions of Use for for-profit users apply.

The CIEA NOG mouse® is produced and distributed under license rights to the following patents and trademarks:
  • Japanese Patent No. 3,753,321
  • US Patent No. 7,145,055; 5,464,764; 5,487,992; 5,627,059; 5,631,153; 5,789,215; 6,204,061; 6,653,113; 6,689,610 EP Patent No. 1,338,198
  • Japanese Trademark Reg. No. 4,823,423
  • US Trademark Reg. No. 3,118,040
  • EU Trademark Reg. No. 3,736,758
hIL15 expression levels: A cohort of 12 female and 10 male hIL15-NOG mice were assayed for hIL15 levels in serum via Luminex assay. hIL15 levels in serum were in the range of ~150-250 pg/mL.