The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Hdac6 exon 2 contains the translation initiation codon.
Exons 11, 12, 13, 14 have been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 11, 12, 13, 14 should result in loss of function of the Hdac6 gene by generating a frameshift (premature STOP codon in exon 16).
The remaining recombination sites are located in intron regions of the genome.
The corresponding constitutive KO allele is available as line 11918.
Datamining and Design performed in 2005.
Gene Family: Deacetylase
Genetic Background: C57BL/6
Gene Symbol: Hdac6
Gene Accession Number: NM_010413
Allele Type: cKO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: histone deacetylase 6