The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 4 and 5 have been flanked by loxP sites (size of loxP-flanked region: 1.5 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 4 and 5 should result in loss of function of the Gpr179 gene by deleting part of transmembrane domains and by generating a frame shift from exon 3 to downstream exons (premature Stop codon in exon 6).
Datamining and Design performed in 2009.
Gene Family: G-protein coupled receptor
Genetic Background: C57BL/6
Gene Symbol: Gpr179
Gene Accession Number: NM_001081220.1
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: G protein-coupled receptor 179