The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 4 to 6 have been flanked by loxP sites (size of loxP-flanked region: 1.7 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 4-6 should result in loss of function of the Galnt2 gene, by removing the 5' half of the catalytic sub-domain A and by generating a frameshift into downstream exons which generates a premature stop codon.
The corresponding conditional KO allele is available as line 10264.
Datamining and Design performed in 2008.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Galnt2
Gene Accession Number: NM_139272.2
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2