Gabrb2 - Model 6511 - PM

Point Mutation Knock Out

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

B6;129S6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 6511
    C57BL/6-Gabrb2tm1.1(b265S)Kwa
  • The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
  • The N265S point mutation was introduced into Gabrb2 exon 8.
  • The neomycin resistance cassette, which is flanked by loxP sites, was introduced into intron 7.
  • Chimeric mice were produced and germ line transmission achieved.
  • The neomycin resistance gene has been removed in the constitutive PM mice by crossing to Cre-Deleter mice.
  • The N265S point mutation in exon 8 abolishes etomidate binding of the Gabrb2 N265S receptor, but keeps the Gabrb2 function otherwise normal.
  • Gabrb2 N265S constitutive PM mice are in a mixed C57BL/6-129SvEv genetic background.
  • See D.S. Reynolds et al, J Neurosci. 2003 Sep 17;23(24):8608-17, for further details.

Gene Family:

Receptor

Genetic Background:

B6:129S6

Gene Symbol:

Gabrb2

Gene Accession Number:

NM_008070

Allele Type:

PM

Source:

Merck Research Laboratories

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

gamma-aminobutyric acid (GABA) A receptor, subunit beta 2

NOTICE: Access to this model is conditioned on the requestor's institution agreeing to the terms and conditions of a Material Transfer Agreement (MTA).

Steps to initiate and place order:

  1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
  2. Nonprofit Investigator inquires on Taconic website.
  3. Taconic will answer questions about the GEM lines and the process.
  4. Taconic sends request for MTA.
  5. Nonprofit Investigator provides scientific workplan answering 4 basic questions.
  6. MTA is issued for Nonprofit Investigator/institution review and signature.
  7. MTA is executed by all parties and release of GEM is approved.
  8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and shipment costs.
  9. Taconic begins cryorecovery upon receipt of PO and delivers GEM line to requestor (See pricing and Deliverables).

It is the requestor's obligation to determine that the GEM meets their research needs.

Learn more about Terms of Use, The Scientific Workplan and Frequently Asked Questions.
Upon execution of the MTA terms of use, requestor needs to provide purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and additional shipment costs.

  1. Taconic will begin standard derivation of cryopreserved material (sperm or embryos) upon receipt of PO from recipient.
  2. Taconic will conduct testing using its International Health Monitoring System™ (IHMS™) to ensure that the mice meet a Murine Pathogen Free™ (MPF™) health status.
  3. Taconic will genotype the recovered mice prior to shipment.
  4. Taconic will provide a health status report, the genotyping results of the recovered animals and genotyping assay information for the requested GEM line.
  5. Taconic shall deliver a minimum of two (2) mice heterozygous at the selected gene of interest of a requested GEM line.
  6. Taconic will make shipping arrangements for the requested GEM to the Licensed Nonprofit recipient.
  7. Nonprofit investigator is responsible for covering the shipment costs.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.