The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
The H101R point mutation was introduced into Gabra1 exon 4.
The neomycin resistance cassette, which is flanked by loxP sites, was introduced into intron 3.
Chimeric mice were produced and germ line transmission achieved.
The neomycin resistance gene has been removed in the constitutive PM mice by crossing to Cre-Deleter mice.
The H101R point mutation in exon 4 abolishes benzodiazepine binding of the Gabra1 H101R receptor, but keeps the Gabra1 function otherwise normal.
Gabra1 H101R constitutive PM mice are in a mixed C57BL/6-129SvEv genetic background.
See R.M. McKernan et al, Nat Neurosci. 2000 Jun;3(6):587-92, for further details.
Gene Family: Receptor
Genetic Background: B6:129S6
Gene Symbol: Gabra1
Gene Accession Number: NM_010250
Allele Type: PM
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1