Floxed Ink4a/Arf Mouse

Conditional Knock Out

Floxed Ink4a/Arf Conditional Knock Out Mouse Model
EZcohort™ Models
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C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 11511-F
    fl/fl
    B6.129P2-Cdkn2atm2Brn/A
  • 11511-M
    fl/fl
    B6.129P2-Cdkn2atm2Brn/A
  • Contains a targeted mutation of Cdkn2a (Ink4a/Arf) which introduced LoxP sites upstream of exon 2 and downstream of exon 3.
  • Cross with the tissue-specific Cre of your choice to develop a tumor model
  • The cell cycle inhibitory protein Cdkn2a is frequently disrupted in various types of human cancer, and germline mutations of this locus can confer susceptibility to melanoma and other tumors.
  • After deletion of the gene via crossing to a tissue-specific Cre line, mice can develop tumors, giving rise to various sarcomas, carcinomas, lymphomas, and metastatic melanoma

Genetic Background:

C57BL/6 Background

Origin:

The Floxed Ink4a/Arf Mouse was developed in 2001 in the laboratory of Dr. Anton Berns of the Netherlands Cancer Institute (NKI). The Cdkn2a mutation was generated by insertion of loxP sites 1 kb upstream of Ink4a exon 2 and 1.5 kb downstream of exon 3. Targeted IB10 (129/Ola) embryonic stem cells were injected into C57BL/6 blastocysts and resulting chimeras were crossed with FVB/N mice. Sibling progeny were crossed to generate Cdkn2a homozygous floxed mice. The line was backcrossed to C57BL/6/J. Taconic received stock in 2011. The line was derived by embryo transfer and was maintained by incrossing mice homozygous for the floxed allele.


Color:

Black

Species:

Mouse

Initial Publication:

Krimpenfort P, Quon KC, Mooi WJ, Loonstra A, Berns A. (2001) Loss of p16Ink4a confers susceptibility to metastatic melanoma in mice. Nature. 413(6851):83-6.



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.