The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic's C57BL/6N Tac ES cell line.
Fam132a exon 1 contains the translation initiation codon.
Exons 1 to 7 have been replaced by the FRT site flanked neomycin resistance gene cassette.
The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
The constitutive KO line has been derived using C57BL/6NTac animals.
Deletion of exons 1 to 7 should result in loss of function of the Fam132a gene.
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2006.
Gene Family: Unknown
Genetic Background: C57BL/6
Gene Symbol: Fam132a
Gene Accession Number: NM_026125
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: family with sequence similarity 132, member A