The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Dpp4 exon 1 contains the translation initiation codon.
Exon 20 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
Deletion of exon 20 should result in loss of function of the Dpp4 gene by deleting the peptidase S9 domain.
The remaining recombination sites are located in intron regions of the genome.
The constitutive KO allele is also available as line 10053.
Data mining and Design performed in 2006.
Gene Family: Peptidase
Genetic Background: C57BL/6
Gene Symbol: Dpp4
Gene Accession Number: NM_010074
Allele Type: cKO
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: dipeptidylpeptidase 4