The targeting vector has been generated using C57BL/6J DNA and transfected into the C57BL/6NTac ES cell line.
Dgat1 exon 1 contains the translation initiation codon.
Exons 3 to 17 have been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of the exons 3 to 17 should result in loss of function of the Dgat1 gene by deleting the MBOAT domain and by removing the 3 ´untranslated region.
The remaining recombination sites will be located in non-conserved regions of the genome.
Datamining and Design performed in 2010.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Dgat1
Gene Accession Number: NM_010046
Allele Type: KO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: diacylglycerol O-acyltransferase 1s