Cyp2c Knockout Mouse

Constitutive Knock Out

Cyp2c Constitutive Knock Out Mouse Model
EZcohort™ Models
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C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 9177-F
    ko/ko
    B6-Cyp2ctm1104Arte
  • 9177-M
    ko/ko
    B6-Cyp2ctm1104Arte
  • This model carries a deletion of fourteen full-length mouse Cyp2c genes
  • Contains a cre-mediated deletion of the following genes in the Cyp2c cluster: Cyp2c55, 2c65, 2c66, 2c29, 2c38, 2c39, 2c67, 2c68, 2c40, 2c69, 2c37, 2c54, 2c50, 2c70.
  • Mouse Cyp2c44, which is physically located outside of the Cyp2c Cluster has not been deleted.
  • In humans, CYP2C enzymes metabolize up to 30% of marketed drugs
  • Useful in dissecting the contribution of the Cyp2c family to total bioavailability of a drug

Genetic Background:

C57BL/6N Background

Origin:

The Cyp2c Knockout Mouse was developed by Taconic in collaboration with CXR Biosciences. The model was created by first replacing mouse exon 1 of Cyp2c55 with a neomycin cassette containing a loxP site and subsequently inserting a hygromycin cassette with a loxP site into intron 6 of the Cyp2c70 gene through gene targeting in C57BL/6NTac-derived ES cells. Transfection of the ES cells with cre recombinase resulted in the deletion of Cyp2c55, 2c65, 2c66, 2c29, 2c38, 2c39, 2c67, 2c68, 2c40, 2c69, 2c37, 2c54, 2c50 and 2c70. The targeted ES cells were injected into BALB/cJBomTac blastocysts. Resultant chimeras were backcrossed to C57BL/6NTac wild type mice. The colony was maintained by mating homozygotes.


Color:

Black

Species:

Mouse

Initial Publication:

Scheer N, Kapelyukh Y, Chatham L, Rode A, Buechel S, Wolf CR. Generation and Characterization of Novel Cytochrome P450 Cyp2c Gene Cluster Knockout and CYP2C9 Humanized Mouse Lines. Mol Pharmacol. 2012 Aug 23.



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.