CIEA NOG mouse®

Constitutive Knock Out / Spontaneous Mutant

CIEA NOG ® Constitutive Knock Out / Spontaneous Mutant Mouse Model

NOD Background

  • Model #
  • Genotype
  • Nomenclature
  • NOG-F
    sp/sp;ko/ko
    NOD.Cg-Prkdcscid Il2rgtm1Sug/JicTac
  • NOG-M
    sp/sp;ko/y
    NOD.Cg-Prkdcscid Il2rgtm1Sug/JicTac
  • Immunodeficient model lacking mature T, B, and NK cells
  • Displays reduced complement activity
  • Has dysfunctional macrophages and dendritic cells
  • Displays no leakiness of T and B cells with increasing age
  • Very low incidence of lymphoma (unlike NOD scid model)
  • Does not develop diabetes
  • Excellent model for a variety of xenograft and human cell engraftment studies
  • Applications in research involving cancer, infectious disease, immunology, regenerative medicine, and humanization
  • The Il2rg gene is sex-linked

Origin:

The CIEA NOG mouse® was developed by Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. The Prkdc scid mutation was identified by Mel Bosma of the Fox Chase Cancer Center in a C.B-17 congenic mouse population. This mutation was backcrossed onto the NOD/ShiJic strain at CIEA for at least eight generations. The Il2rg targeted mutation was developed by Dr. Kazuo Sugamura of Tohoku University by targeting the gene in ES cells derived from a 129 strain. Portions of exons 7 and 8 were replaced with a neo cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Resultant chimeras were backcrossed onto the C57BL/6JJic background for at least eight generations. The CIEA NOG mouse® was developed by backcrossing the C57BL/6JJic-Il2rg line to the NOD/ShiJic-Prkdc scid line for a total of eight generations. Taconic received stock in 2006, and the line was derived through embryo transfer. The CIEA NOG mouse® colony was refreshed in 2011 and 2015 via rederivation of new stock using donors/donor material sourced from the CIEA NOG mouse® foundation colony at CIEA. The mice are maintained by breeding females homozygous for both the Prkdc scid and Il2rg mutant alleles with males that are homozygous for the Prkdc scid allele and hemizygous for the Il2rg mutant allele.


Availability:

Available now*
*Please note that advance order is encouraged for order of male mice. There is a minimal 4-week advance notice required on orders of male older than 3 weeks of age.

Color:

Albino

Species:

Mouse

Initial Publication:

Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, Heike T, Nakahata T. (2002) NOD/SCID/γ mouse: an excellent recipient mouse model for engraftment of human cells. Blood 100(9):3175-3182.



Nonprofit users (excluding users at nonprofit foundations which are affiliated with a for-profit entity): For internal research purposes, the CIEA NOG mouse® Conditions of Use for nonprofit users apply. If you wish to perform sponsored research or fee-for-service contract research using the CIEA NOG mouse®, please inquire for access conditions

For-profit users and users at foundations which are affiliated with for-profit entities: The CIEA NOG mouse® Conditions of Use for for-profit users apply.

Does your research require the use of neonates? Please contact us for more information on neonate access options.

The CIEA NOG mouse® is produced and distributed under license rights to the following patents and trademarks:
  • Japanese Patent No. 3,753,321
  • US Patent No. 7,145,055; 5,464,764; 5,487,992; 5,627,059; 5,631,153; 5,789,215; 6,204,061; 6,653,113; 6,689,610
    EP Patent No. 1,338,198
  • Japanese Trademark Reg. No. 4,823,423
  • US Trademark Reg. No. 3,118,040
  • EU Trademark Reg. No. 3,736,758
For some applications, weight may be critical. For orders where weight is critical, please place orders by weight, not by age. Taconic can accept orders by weight for this model, except at the DF health status. A minimum 3 gram span is required. All weights are weights at time of packing. Mice and rats can lose weight in transit. Orders for specific weight spans will be assessed a nominal fee. More detail on weight order policies is available.

n= 100 animal per sex Restricted Flora health standards from US colonies. Data collected 2012-2013.
High and Low represent mean +/- standard deviations.
The sample size above is not large enough to necessarily represent a normal population.
All growth curves represent animals housed in our barriers, at our standard density and fed NIH31-M diet. Variations at customer facilities will alter expected growth curves.
Growth charts are provided only as a guide, if a specific weight criteria is needed please order animals by weight.

Customize this chart by clicking the legend elements, then explore download options by hovering your cursor over the down arrow to the right of the chart title.

n= 25 animal per sex Restricted Flora health standards from US colonies. Data collected 2013.
The sample size for this study is too small for calculation of a standard deviation to be meaningful.
All animals were fed NIH-31M and housed at densities appropriate for their age.
Growth charts are provided only as a guide, if a specific weight criteria is needed please order animals by weight.

Customize this chart by clicking the legend elements, then explore download options by hovering your cursor over the down arrow to the right of the chart title.
Average litter size: 7