The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 3 to 5 have been flanked by loxP sites (size of loxP-flanked region: 2.0 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 3 to 5 should result in loss of function of the Chk1 gene by generating a frameshift.
Datamining and Design performed in 2004.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Chk1
Gene Accession Number: NM_007691
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: checkpoint kinase 1 homolog (S. pombe)