Humanized CAR Mouse

Targeted Replacement

Humanized CAR Targeted Replacement Mouse Model
This line is cryopreserved and requires cryorecovery.

  • Model #
  • Genotype
  • Nomenclature
  • 9101-F
    hu/hu
    C57BL/6-Nr1i3tm1(NR1I3)Arte
  • 9101-M
    hu/hu
    C57BL/6-Nr1i3tm1(NR1I3)Arte
  • This targeted mutation strain carries a replacement of the mouse Nr1i3 gene which encodes for the nuclear receptor CAR with the corresponding human orthologous gene.
  • Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity, and efficacy in humans.

Origin:

The Humanized CAR Mouse was developed by Taconic in collaboration with CXR Biosciences. It was created through a knock in of a human CAR construct containing the genomic sequence from the translational start site on exon 2 to exon 9 onto the ATG of murine Car in C57BL/6NTac-derived ES cells. The expression of the human CAR receptor is driven by the mouse Car promoter. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate the selection marker. Taconic received stock in 2009, and the line was embryo transfer derived. The colony was maintained by mating homozygotes.


Color:

Black

Species:

Mouse

Initial Publication:

Scheer N, Ross J, Rode A, Zevnik B, Niehaves S, Faust N, Wolf CR. (2008) A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response. J. Clin. Invest. 118(9): 3228-3239.



This line is available for immediate cryorecovery. A limited breeding agreement with a license fee is required. Cryorecovery fees are additional.