Brd4 - Model 13231 - cKO

Conditional Knock Out

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 13231
    C57BL/6-Brd4tm1.1Mrl
  • The targeting strategy allows the generation of a conditional Knock-Out (KO) and a constitutive KO of the Brd4 gene.
  • The targeting strategy is based on the NCBI transcript NM_020508.3.
  • Exon 3 contains the translation initiation codon.
  • Exons 6 and 7 have been flanked by loxP sites (size of loxP-flanked region is 1.8 kb).
  • The positive selection marker (Puromycin resistance - PuroR) has been flanked by FRT sites and inserted into intron 5.
  • The targeting vector has been generated using BAC clones from the C57BL/6J RPCIB-731 BAC library and will be transfected into the TaconicArtemis C57BL/6N Tac ES cell line.
  • Homologous recombinant clones will be isolated using positive (PuroR) and negative (Thymidine kinase - Tk) selections.
  • The conditional KO allele is obtained after Flp-mediated removal of the selection marker.
  • The constitutive KO allele is obtained after Cre-mediated recombination. Deletion of exons 6 and 7 should result in the loss of function of the Brd4 gene by generating a frameshift from exon 5 to exons 8-11 (premature Stop codon in exon 8). In addition the resulting transcript may be a target for Non-sense Mediated RNA Decay and may therefore not be expressed at significant level.
  • The remaining recombination sites will be located in non-conserved regions of the genome.

Gene Family:

Transcriptional regulator

Genetic Background:

C57BL/6

Gene Symbol:

Brd4

Gene Accession Number:

ENSMUST00000003726

Allele Type:

cKO

Source:

TaconicArtemis

Species:

Mouse

Official Gene Name:

bromodomain containing 4

NOTICE: Access to this model is conditioned on the requestor's institution agreeing to the terms and conditions of a Material Transfer Agreement (MTA).

Steps to initiate and place order:

  1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
  2. Nonprofit Investigator inquires on Taconic website.
  3. Taconic will answer questions about the GEM lines and the process.
  4. Taconic sends request for MTA.
  5. Nonprofit Investigator provides scientific workplan answering 4 basic questions.
  6. MTA is issued for Nonprofit Investigator/institution review and signature.
  7. MTA is executed by all parties and release of GEM is approved.
  8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and shipment costs.
  9. Taconic begins cryorecovery upon receipt of PO and delivers GEM line to requestor (See pricing and Deliverables).

It is the requestor's obligation to determine that the GEM meets their research needs.

Learn more about Terms of Use, The Scientific Workplan and Frequently Asked Questions.
Upon execution of the MTA terms of use, requestor needs to provide purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and additional shipment costs.

  1. Taconic will begin standard derivation of cryopreserved material (sperm or embryos) upon receipt of PO from recipient.
  2. Taconic will conduct testing using its International Health Monitoring System™ (IHMS™) to ensure that the mice meet a Murine Pathogen Free™ (MPF™) health status.
  3. Taconic will genotype the recovered mice prior to shipment.
  4. Taconic will provide a health status report, the genotyping results of the recovered animals and genotyping assay information for the requested GEM line.
  5. Taconic shall deliver a minimum of two (2) mice heterozygous at the selected gene of interest of a requested GEM line.
  6. Taconic will make shipping arrangements for the requested GEM to the Licensed Nonprofit recipient.
  7. Nonprofit investigator is responsible for covering the shipment costs.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.