B6 Albino A++

Targeted Replacement

B6 Albino A++ Targeted Replacement Mouse Model
This line is cryopreserved and requires cryorecovery.

C57BL/6NTac Background

  • Model #
  • Genotype
  • Nomenclature
  • 11227-F
    C57BL/6NTac-Atm1.1Arte Tyrtm1Arte
  • 11227-M
    C57BL/6NTac-Atm1.1Arte Tyrtm1Arte
  • A new and improved B6 albino strain based on the C57BL/6NTac substrain.
  • Contains two mutations, a mutation in the Tyr gene to introduce albinism, and a reversion of the non-agouti (a) locus to the agouti locus (A)
  • Using the Taconic B6 Albino as a blastocyst host and mating the subsequent chimeras to a standard B6 mouse permits detection of ES cell germline transmission by coat color and ensures that the G1 animal is identical to the B6 genome at all coat color loci
  • The Taconic B6 albino is an ideal host for maintaining substrain specificity ES cells derived from the C57BL/6NTac substrain
  • Can also be used as a genetic background for models in which a B6 background is needed with an albino coat, for example in imaging applications
  • Available only from Taconic!
  • Please note that chimeras generated using B6-derived ES cells and blastocysts from the B6 Albino A++ will be tri-colored. The agouti signalling peptide (produced in blastocyst-derived cells, which appear albino in the chimera) can produce a paracrine signalling effect in some cells derived from the ES cells. ES cell-derived hair patches can thus be either black or agouti. Both black and agouti patches should be counted for determination of chimerism percentages.

Genetic Background:

C57BL/6NTac Background


The B6 Albino A++ model was developed by Taconic. In C57BL/6 mice, intron 1 of the agouti locus contains a retrotransposon which is responsible for the non-agouti phenotype. In the Taconic B6, the region containing this retrotransposon was replaced with a FRT-flanked neomycin-puromycin resistance cassette in C57BL/6NTac-derived ES cells via gene targeting. The constitutive knock out allele was then generated via Flp-mediated removal of the selection cassette. The Tyr mutation is a constitutive knock in of a point mutation in which a G nucleotide located at position 369 in exon 1 has been replaced with C nucleotide, resulting in an amino acid substitution at position 103 (Cys>Ser). The F3 site flanked selection marker was removed by via Flp recombinase. Mice containing the agouti and tyrosinase mutations were intercrossed, and then the line was intracrossed to homozygosity.


Use of the Taconic B6 Albino A++ in the following manner permits detection of ES cell germline transmission in a single step by coat color, with NO genotyping required!

Additionally, the germline progeny are identical to the C57BL/6 genome at all coat color loci!





Initial Publication:

Zevnik B, Uyttersprot NC, Perez AV, Bothe GW, Kern H, Kauselmann G. (2014) C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS One. 2014 Mar 5;9(3):e90570.   

This line is available for immediate cryorecovery. A limited breeding agreement with a license fee is required. Cryorecovery fees are additional