The Ahr Knockout mouse was developed by Taconic in collaboration with CXR Biosciences. It was generated by crossing an AHR humanized mouse line (Model 9165) with a Cre deleter mouse. The Humanized AHR Mouse model was created through a targeted replacement of mouse Ahr Exon 3 with a cDNA construct containing human AHR Exons 3-11 in C57BL/6NTac-derived ES cells. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate selection markers. The Ahr Knockout Mouse model was derived from the Humanized AHR Mouse line through a Cre-mediated deletion of the human AHR sequence. Mouse exon 3 is deleted in the knockout, resulting in an out of frame splicing of exons 2 to exon 4. The absence of functional Ahr protein was proven experimentally. Taconic received stock in 2009, and the line was embryo transfer derived. The colony is maintained by mating homozygotes.
Not published yet.
Label License: Conditions of Use - Model 9166