Humanized AHR Mouse

Targeted Replacement

Humanized AHR Targeted Replacement Mouse Model
This line is cryopreserved and requires cryorecovery.

  • Model #
  • Genotype
  • Nomenclature
  • 9165-F
    hu/hu
    C57BL/6-Ahrtm1.1(AHR)Arte
  • 9165-M
    hu/hu
    C57BL/6-Ahrtm1.1(AHR)Arte
  • This targeted mutation strain carries a replacement of the mouse aryl hydrocarbon receptor (Ahr) gene with the corresponding human orthologous gene.
  • Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity, and efficacy in humans.

Origin:

The Humanized AHR Mouse was developed by Taconic in collaboration with CXR Biosciences. It was created through a targeted replacement of mouse Ahr Exon 3 with a cDNA construct containing human AHR Exons 3-11 in C57BL/6NTac-derived ES cells. This leads to the expression of a fusion protein of amino acids encoded by mouse Exons 1 and 2 and human Exons 3 to 11 driven by the mouse Ahr promoter. The amino acids encoded by Exon 1 and 2 of mouse and human AHR are highly conserved, with only two changes in Exon 1. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate the selection marker. Taconic received stock in 2009, and the line was embryo transfer derived. The colony was maintained by mating homozygotes.


Color:

Black

Species:

Mouse

Initial Publication:

Not published yet.



This line is available for immediate cryorecovery. A limited breeding agreement with a license fee is required. Cryorecovery fees are additional.