The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 3 to 7 have been flanked by loxP sites (size of loxP-flanked region: 2.0 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 3-7 should result in loss of function of the Acss2 gene by deleting the transmembrane domain and part of the catalytic domain and by generating a frameshift from exon 2 to downstream exons (premature Stop codon in exon 8).
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Acss2 gene:
- The Ggtl3 gene (Entrez ID: 207182; ENSMUSG00000027603) is located approx. 34 kb upstream of Acss2 exon 3 in a head-to-head orientation.
- The Gss gene (Entrez ID: 14854; ENSMUSG00000027610) is located approx. 13 kb downstream of Acss2 exon 7 in a tail-to-tail orientation.
Datamining and Design performed in 2009.
Gene Family: Synthetase
Genetic Background: C57BL/6
Gene Symbol: Acss2
Gene Accession Number: NM_019811.3
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: acyl-CoA synthetase short-chain family member 2