Comprehensive Phenotypic Data Packages

Neurological Disorders: Inverted Screen

The Inverted Screen test is used to measure motor strength/coordination. Untrained mice are placed individually on top of a square (7.5 cm x 7.5 cm) wire screen which is mounted horizontally on a metal rod. The rod is then rotated 180° so that the mice are on the bottom of the screens. The following behavioral responses are recorded over a 1 min testing session: fell off, did not climb, and climbed up.

A motor strength deficit is apparent when there is a 50% point difference between homozygous and wild type mice for the fell down response. A motor impairment is also indicated by 0/8 or 1/8 homozygous mice failing to climb up (data not shown). Enhanced motor coordination is indicated by high ratios, 7/8 or 8/8, of homozygous mice climbing up on the inverted screen.

These data are presented in table format as illustrated below.

Genotype Ratio Fell Down Percent Fell Down Ratio Climbed Up Percent Climbed Up
Homozygous (n=8) 0/8 0.0 4/8 50.0
Wild Type Littermates (n=8) 0/8 0.0 4/8 50.0
Wild Type Historical (n=10,000)   3.7   60.0