Comprehensive Phenotypic Data Packages

Cardiovascular Disease: Bleeding Time

Bleeding time is an in vivo assay indicative of platelet and coagulation function. The bleeding time assay determines whether a particular knockout line has any deficiency with respect to platelet function or coagulation. Mice are anesthetized with isoflurane and then 7 mm of their tail is transected completely. The tail is placed into 37°C saline and the time to bleeding cessation is measured. The maximum time allowed is 5 minutes. At that point the tail is cauterized and the mouse is recovered.

Data are presented in table format:

Genotype 0-1 Min 1-2 Mins 2-3 Mins 3-4 Mins 4-5 Mins 5+ Mins Ratio Long Bleed % Long Bleed
Homozygous 1 2 2 0 0 1 1/6 17%
Wild Type 1 2 2 0 1 2 2/6 25%
Heterozygous 0 0 0 0 0 0 0/0 0