Knockout QC Data for Gene Trapped Lines

An example of the RT-PCR analysis performed on the non-lethal gene trapped lines is shown below. Primers complementary to exons flanking the insertion site are used in an RT-PCR reaction to determine whether the gene trapping vector has effectively disrupted transcription of the endogenous message in homozygous mice.

RT-PCR Analysis
Three samples were run for each tissue type under the following conditions:
  • Tissue in the absence of gene specific reverse transcript (RT) and Actin control primers.
  • Tissue in the presence of RT and the absence of Actin primers.
  • Tissue in the presence of RT and Actin primers.