Phenotypic Data Packages

Understanding the phenotype associated with a targeted or gene trapped mutation is essential for identifying and selecting drug targets or studying symptoms of human diseases. Taconic enables you to jumpstart your research by offering off-the-shelf phenotypic data packages for all of the KO Repository lines. These phenotypic data were collected at the time that the line was generated. Cohorts of animals were developed and tested in a comprehensive battery of assays that were fully validated with benchmark knockouts and/or drugs to confirm their reliability and reproducibility. Phenotypic assays focused on 6 main disease areas:
  • Cancer
  • Cardiovascular Disease
  • Immune System Disorders
  • Metabolic Disorders
  • Neurological Disorders
  • Ophthalmology
  • For assays that were not sex-specific, equal numbers of males and females were tested.
  • For non-lethal homozygous lines the following number of each genotype were assessed:
    • 2-4 wild type
    • 0-4 heterozygotes
    • 4-8 homozygotes
  • For histological analyses a maximum of 2 animals per group were assessed. Only remarkable findings are reported.
  • For lethal homozygous lines the following number of each genotype were assessed:
    • 2-4 wild type
    • 4-8 heterozygotes
  • For histological analyses a maximum of 2 animals per group were assessed. Only remarkable findings are reported.
Lines were created by injecting 129S5 ES cells into C57BL/6J-Tyrc-Brd blastocysts. Resulting mice were then bred with B6129F1 hybrids to maintain a mixed 129S5 x C57BL/6J-Tyrc-Brd background.