Transgene Expression

Transgene Expression in Mice or Rats

Transgene expression in mice or rats can help when you’re exploring gene function or engineering new pathways. Taconic Biosciences’s scientists are ready to generate your transgene-expressing small animal model, and have constitutive, conditional, or inducible expression options depending on your study goals.
Targeted Transgenesis:

Taconic generates Targeted Transgenic mouse models by site-specific recombination between an exchange vector and the ROSA26 docking site in ES cells. A conditional version of Targeted Transgenesis can be generated by the insertion of a CAG promoter and a strong transcriptional termination site, flanked by LoxP sites, 5' to your gene of interest.

  • Insert your murine or human gene of interest into the well-defined ROSA26 locus without interfering with other gene expression.
  • Overexpress your gene of interest in a conditional fashion.
  • Generate only one well-defined founder line.
  • Achieve high transgene expression levels by using the strong CAG promoter.
Targeted Transgenic Allele
Conditional Targeted Transgenesis:

Overexpress transgenes in a tissue-specific manner in combination with the appropriate Cre-expressing mouse line. Conditional targeted transgenesis is also your ideal tool if you work with transgenes that can adversely affect mouse development.
Conditional Targeted Transgenesis Allele
Random Transgenesis by Pronuclear Microinjection:

Taconic's Custom Transgenics Program employs pronuclear microinjection technology to generate genetically-modified mice or rats transgenic founders. With pronuclear microinjection, a pre-designed gene (or genes) are injected, or co-injected, and then randomly integrated into the mouse or rat genome, with either single or multiple transgene copies at the site of insertion (i.e., gene dosage effect).
Targeted Transgenic Production Process


Detailed Milestone Reports
More Facts About Taconic's Custom Transgenic Program:

  • Taconic has generated more than 10,000 founder mice (and/or rats) from over 3,500 transgene constructs.
  • Taconic has an overall success rate of 98% in generating transgenic mice/rats.
  • Taconic's transgenic mice/rats have been used within the global scientific community to study the biology of gene function(s) and establish numerous animal models of human disease.
Recombinase Mediated Cassette Exchange (RMCE) Efficiency

(RMCE) Efficiency
Random Transgenesis - Pronuclear Microinjection

Pronuclear Microinjection
Depending upon our client's needs, Taconic's transgenic mouse program can employ a variety of mouse strains for transgenic production. These include:

Standard Strains: Special Strains:
FVB/NTac C57BL/6NTac
B6SJL (F2 hybrid) C57BL/6J
Other strains upon request
For our clients wishing to develop transgenic rats, we are able to offer the following rat strains:

Standard Strain: Special Strains:
Sprague Dawley® Lewis
Fischer 344
Long Evans
Other strains upon request
Packages include:

  • Documentation and verification of PCR-based genotype protocol by Taconic Molecular Analysis.
  • Coordinated transfer of donor males to Taconic's breeding facility in Germantown, NY.
  • Confirmation of genotype of donor males by PCR-based genotyping.
  • Rapid Expansion using C57BL/6NTac donor females.
  • Holding of excess donor males until offspring from rapid expansion are weaned.
  • Initial post-derivation comprehenisve health testing (IHMS™-52).
  • PCR based genotyping of derived offspring.
  • Holding of derived pups from weaning until ready for shipment (4 weeks) at the Isolator Breeding Solutions facility.
  • Initial cohort of animals ready for shipment beginning at 5 weeks of age.
    • Large package 18-20/sex/genotype
    • Small package 8-10/sex/genotype
    • Genotypes include heterozygous and wild type

Additional package details:

  • Package ends when initial cohort is ready for shipping. Post package breeding and fees will be based on your breeding plan.
  • Package includes initial health testing only, ongoing health testing can be done for an additional charge.
  • Shipping charges from Taconic to customer are not included in package.
  • Package assumes receipt of 4 donor males from Taconic, additional services will be charged at catalog prices.
  • Pricing assumes genetic modification is not sex linked, Mendelian ratios will be obtained, 80% of female breeders will deliver live pups that survive until weaning, and an average litter size of 5 pups. Additional charges may apply if these conditions are not met.
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