Precision Editing of Native Genes

CRISPR Gene Editing of Mouse and Rat Models

After thorough evaluation of the technology through a number of pilot projects, Taconic Biosciences now offers CRISPR (Clustered Regularly Interspersed Palindromic Repeats) gene editing to provide clients with an efficient way to generate custom mouse and rat models and data for their research. Applications include studies of gene function in vivo and generation of models of human disease.

CRISPR gene editing empowers Taconic to provide clients with a technology that speeds timelines, reduces costs, and improves efficiencies in custom model generation. Gene knockouts and insertions of point mutations are made by direct injection into fertilized mouse oocytes leading to shorter timelines than traditional gene targeting method for generating founder mice. Beyond those advantages, CRISPR gene editing can be used to re-target existing repository mouse models or client proprietary models. Genetic modifications of well-established existing models using CRISPR may provide a fast and cost-efficient solution for generating specialized models, especially if multiple modified alleles are already present.

As a global leader in custom model generation technologies for over two decades, Taconic consults with its clients to select the most appropriate technology for its custom model generation needs. Contact us to talk with a Taconic scientist about how CRISPR technologies can be leveraged for your custom model generation needs.

The CRISPR/Cas9 System

CRISPR How It Works
Generating a genome-edited mouse or rat is a multi-step process that involves:

  • Selection of target site, assays, and oligonucleotides, as well as predicted off-target sites
  • Preparation of sgRNA and Cas9 mRNA
  • Injection of sgRNA and Cas9 mRNA into mouse C57BL/6NTac zygotes, to generate F0 mutated candidate animals
  • Genotyping and target-site sequence analysis in mutated candidate F0 animals
  • Optional steps
    • Germline breeding of F0 founder animal (optional)
    • Sequence analysis of germline transmitted F1 animals (optional)
    • Off-target analysis in germline transmitted F1 animals (optional)