Gene Expression Analysis

Gene Expression Analysis is used to determine whether a gene is expressed and can also determine in which tissues gene expression is turned on. This is important in the initial characterization of genetically engineered mice and rats and can be used as a quality control assay for ongoing monitoring of genetically engineered mice and rats.

Taconic offers the following gene expression services:

Gene Expression Assay Design

  • Design and validation of a quantitative reverse transcriptase real-time PCR (RT_PCR) assay
  • Used to assess the mRNA levels of a single gene in a particular tissue

Gene Expression Analysis

  • Quantitative reverse transcriptase real-time PCR (RT-PCR) assay to assess the mRNA levels of single gene in a particular tissue
  • Appropriate for the evaluation fo expression of a transgene in tissue(s) of interest
  • Appropriate for evaluation of expression of endogenous gene in response to a genetic manipulation in tissue(s) of interest

FACS Analysis (Dual-Antibody)

  • Evaluation of protein expression in blood samples
  • Appropriate for verification of protein expression on founders and/or breeders for lines that express a transgene in blood