• Model #
  • Zygosity
  • Nomenclature
  • FVB-F
    FVB/NTac
  • FVB-M
    FVB/NTac
  • Pronuclei of FVB embryos are easily manipulated for microinjection of transgenes
  • High reproductive performance and excellent nurturing characteristics
  • Males tend to fight when group housed
 
Origin: Taconic received stock at F59 from NIH Animal Genetic Resource in 1998. The mice were derived by embryo transfer. Taconic's FVB mice originated from NIH at F30 prior to 1999. FVB is a substrain of N:GP Swiss mouse which was generated at NIH in 1935. A second colony was generated for Pertussis Research and designated N:NIH(S). The mice from this colony were backcrossed eight generations (F8) and designated HSFS/N. FVB, Friend Leukemia Virus, strain B was selected for from the HSFS/N colony. The Taconic foundation colony was at F83 in 2005.

Genetics:
  • Strain Profile: Acy1s, Akp1b, Amy1a, Apoa1b, Car2b, Ce2a, Es1b, Es3c, Esda, Es11a, Fv1b, H6pd1b, Ggca, Glo1a, Got2a, Gpi1b, Gr1a, Gus-sb, Hbac, Hbbd, Idh1a, Ldr1b, Mod1a, Mpi1b, Mup1c, Neu1b, Pep3b, Pgm1a, Pgm2a, Pnpa, Sod1a, Trfb
  • Immunology: H2q
  • Other: Cd5b, Cd8ab, Cd8bb, Hc1, Thy1a
  • Retinal Degeneration: Pde6brd1
 

Coat Colour Loci:
Tyrc

Color: Albino


FVB Physiological Data Summary (April 2003; N=10/group):

Parameter Units FVB Males FVB Females
Serum Chemistry avg ± S.D.    
Calcium mg/dL 11.0 ± 0.5 10.7 ± 0.3
Phosphorous mg/dL 10.5 ± 1.5 10.3 ±1.9
Glucose mg/dL 192 ± 25 156 ± 20
Creatinine mg/dL 0.1 ± 0 0.1 ± 0
BUN mg/dL 22 ± 2 19 ± 4
Total Bilirubin mg/dL 0.1 ± 0 0.1 ± 0
ALK U/L 175 ± 12 248 ± 56
ALT U/L U/L 35 ± 12 47 ± 51
Total Protein g/dL 5.9 ± 0.1 5.6 ± 0.2
Blood Counts
Erythron      
Red Blood Cells X10^6/uL 10.317 ± 0.335 9.89 ± 0.44
Nucleated RBC X10^6/uL 0 0
Hemoglobin g/dL 15.7 ± 0.5 15.6 ± 0.7
Hematocrit % 50.2 ± 1.3 50.4 ± 2.2
MCV fL 49 ± 1 51 ± 1
MCH pG 15.2 ± 0.5 15.8 ± 0.2
MCHC % 31.3 ± 1.0 31.0 ± 0.4
Platelets      
Platelets X10^3/uL 1442 ± 124 1616 ± 184
Leukogram      
White Blood Cells x10^3/uL 5.28 ± 1.32 6.97 ± 2.37
Neutrophil x10^3/uL 0.495 ± 0.315 0.673 ± 0.446
Bands x10^3/uL 0 0
Lymphocyte x10^3/uL 4.709 ± 1.209 6.115 ± 2.043
Monocytes x10^3/uL 0.067 ± 0.076 0.135 ± 0.125
Eosinophil x10^3/uL 0.013 ± 0.027 0.047 ± 0.046
Basophils x10^3/uL 0 0
Others x10^3/uL 0 0
Organ Weights      
Stomach g 0.278 ± 0.028 0.243 ± 0.026
Ileum g 0.972 ± 0.136 0.811 ± 0.086
Colon g 0.119 ± 0.076 0.124 ± 0.071
Lungs g 0.246 ± 0.041 0.181 ± 0.019
Heart g 0.157 ± 0.028 0.116 ± 0.017
Liver g 1.286 ± 0.085 0.968 ± 0.147
Spleen/Pancreas g 0.278 ± 0.030 0.206 ± 0.037
Kidney (L) g 0.176 ±0.014 0.122 ± 0.011
Kidney (R) g 0.205 ± 0.059 0.127 ± 0.015
Testes (L) g 0.100 ± 0.008 -
Testes (R) g 0.100 ± 0.008 -
Ovary (L) g - 0.009 ± 0.002
Ovary (R) g - 0.011 ± 0.004>
Urinalysis      
Glucose mg/dL Neg. Neg.
Blood - Neg. Neg.
Ph - 6.1 ± 0.2 6.2 ± 0.4
Protein mg/dL 30 Trace
Specific Gravity - 1.03 ± 0 1.02 ± 0.01

Phenotyping Procedures:

Animal Receipt and Maintenance

A quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line FVB (FVB/NTac), age 7 weeks. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH #31M rodent diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1-10, females 11-20).

 

Urinalysis

All mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer's instructions, and the results are presented in Table 1. Mouse #19 gave an unusually large amount of urine (15ml). This mouse was markedly larger than the other females, but no abnormalities were noted.

 

Clinical Chemistry and Hematology

A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood, and slides were delivered to LabCorp (RTP, NC) for analysis; the results are presented in Table 2. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually.

 

Necropsy and Organ Weights

All mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. Tabulation of organ weights is presented in Table 3. The pathologist's summary and detailed histological descriptions follow.

 

Discussion

All animals were thrifty on arrival and appeared clinically normal. Locomotor behavior was normal and there were no visible lesions or discharges at the mucous membranes. All tissues appeared normal on gross necropsy evaluation and weights were within normal limits. There were no unusual findings upon collection of these tissues nor otherwise observed in the body cavities.

Mouse #19 was markedly larger than the other females. Internal organs were proportionately larger as well. No other abnormalities were noted. The overall profile of these mice is consistent with expectations of a general-purpose mouse. None of the parameters are indicative of impaired system function.